Journal: Hypertension

Article Title: Identification of Bona Fide Alternative Renin Transcripts Expressed Along Cortical Tubules and Potential Roles in Promoting Insulin Resistance In Vivo Without Significant Plasma Renin Activity Elevation

doi: 10.1161/hypertensionaha.114.03394

Figure Lengend Snippet: Figure 7. A, The results of quantitative reverse transcription polymerase chain reaction analyses of inflammatory molecules in heart, kidney, and liver of both ARen2TG and wild-type littermates are shown as relative abundance, using GAPDH expression as an internal control. *, **, #, ##Significantly elevated compared with the expressions of wild-type littermates. Repeated measure ANOVA and subsequent post hoc analyses were performed. B, Representative results of in situ histological examinations for livers of both transgenic mice and wild-type littermate using rabbit polyclonal anti-angiotensin II antibodies (NBP1-30027; Novus Biologicals, LLC, Littleton, CO) were shown. a–c, Enhanced immunohistochemical staining in livers of transgenic mice compared with those of wild-type littermates (Bd), suggesting enhanced angiotensin II production in the organs.

Article Snippet: We performed experiments to compare ARen2TG mice with wild-type littermates in terms of following phenotypes: measurement of blood pressure,16,17 measurement of plasma renin activity,16,18,19 the results of in vivo near-infrared imaging using the renin substrate analog ReninSense 680 FAST (Perkin Elmer, Waltham, MA),20 the results of insulin tolerance tests,21 fasting plasma glucose levels, the expressions of inflammatory molecules in various tissues on quantitative RT-PCR analyses,16,18,19 and in situ histological examinations for livers of both transgenic mice and wild-type littermate using rabbit polyclonal anti-angiotensin II antibodies (NBP1-30027; Novus Biologicals, LLC, Littleton, CO).22,23 All experiments were performed according to the protocols of manufacturer, the references, and our reports as specified above.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Expressing, Control, In Situ, Transgenic Assay, Immunohistochemical staining, Staining

Journal: Cells

Article Title: Loss of Endothelial Glycocalyx Hyaluronan Impairs Endothelial Stability and Adaptive Vascular Remodeling after Arterial Ischemia

doi: 10.3390/cells9040824

Figure Lengend Snippet: Loss of hyaluronan in ECs decreases angiopoietin 1 presence in ECs. ( A ) Representative images of angiopoietin 1 (Ang1, top) staining and BS1-lectin staining (middle) for ECs localization (scale bar = 20 µm) and ( B ) quantification of endothelial associated Ang1 in ligated and non-ligated gastrocnemius muscle of Has2-cKO ( n = 5) and control ( n = 3) mice. ( C ) Representative overlay images of platelet-derived growth factor receptor beta (PDGFRβ, green), laminin (red) and co-staining (yellow) in ligated and non-ligated gastrocnemius muscle of Has2-cKO ( n = 5) and control ( n = 3) mice (scale bar = 20 µm). ( D ) Representative overlay images of vascular endothelial growth factor 165 (VEGF165, green), BS1-lectin (red) and ( E ) quantification of VEGF165 and EC area ratio in ligated and non-ligated gastrocnemius muscle of Has2-cKO ( n = 5) and control ( n = 3) mice (scale bar = 20 µm). Values are given as mean ± SD. * p < 0.05.

Article Snippet: Primary Goat anti-mouse Ang1 (AF923, R&D Systems, Minneapolis, MN, USA), rabbit anti laminin (Z0097, Dako), rat anti-mouse PDGFβ (ab51876, Abcam, Cambridge, UK), HA binding peptide Neurocan-dsRed [ ] (200 μg/mL), or lectin from Bandeiraea simplicifolia isolectin B4 (BS-1-TRITC, L5264, Sigma or BS-1-BIOTIN, L2140, Sigma, Zwijndrecht, the Netherlands) were incubated overnight at 4°C in blocking buffer, followed by an appropriate secondary antibodies for 1 h at room temperature.

Techniques: Staining, Control, Derivative Assay

Journal: Frontiers in Genetics

Article Title: The impact of decreased expression of SVEP1 on abnormal neovascularization and poor prognosis in patients with intrahepatic cholangiocarcinoma

doi: 10.3389/fgene.2022.1127753

Figure Lengend Snippet: SVEP1 expression is positively related to the expression of Ang-1, which plays an important role in the maintenance of vascular stability. (A) Representative IHC images of Ang-1 in ICC tissues. (B) IHC image quantification results. Relative proportions of high and low levels of Ang-1 in the group of tumor tissues with high and low expression of SVEP1, and the correlation analysis between Ang-1 and SVEP1. (C–D) Correlation of Ang-1 expression with the OS (C) and DFS (D) time of ICC patients, respectively.

Article Snippet: The primary antibody included rabbit anti-SVEP1 polyclonal antibody (Abcam, cat#ab121677), rabbit anti-Angiopoietin-1 (Ang-1) polyclonal antibody (Bioss, cat#bs-0800R), and mouse anti-Ki-67 monoclonal antibody (Zhongshan Goldbridge Biotechno-logy CO., Ltd., Beijing, China).

Techniques: Expressing